RFWD3 polyclonal antibody

Product Specifications

Background

E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

E3 ubiquitin-protein ligase RFWD3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201; RING-type E3 ubiquitin transferase RFWD3; RFWD3; RNF201

Swiss Prot

Q6PCD5

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 380-430 of Human RFWD3 .

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 85 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RFWD3 polyclonal antibody detects endogenous levels of RFWD3 protein.

Applications Notes

Western blot (WB) analysis of RFWD3 polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:SP2/0 whole cell lysate (40ug)