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CA4 polyclonal antibody

Product Specifications

Background

Carbonic anhydrase IV (CA IV) is glycosylphosphotidylinositol-anchored to the outer surface of the plasma membrane where it catalyzes hydration-dehydration of CO2/HCO3 - . CA IV is present on the plasma face of microcapillaries and in the choriocapillaris of the human eye. CA IV facilitates renal acidification in the kidney and is responsible for the regulation of interstitial pH (pHo) transients in brain. Impairment in targetting leads to disruption of HCO3 - secretion and associates with malfunction in cystic fibrosis cells. Carbonic anhydrases are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases show extensive diversity in tissue distribution and in their subcellular localization.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Carbonic anhydrase 4; Carbonate dehydratase IV; Carbonic anhydrase IV; CA-IV; CA4

Swiss Prot

P22748

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 120-170 of Human CA4.

Conjugation

Unconjugated

Applications

WB

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 35 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CA4 polyclonal antibody detects endogenous levels of CA4 protein.

Applications Notes

Western blot (WB) analysis of CA4 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug)

Host or Source

Rabbit

Available Sizes

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