TUBA3C/TUBA3D/TUBA3E polyclonal antibody

Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and ε Tubulin. α and β tubulins form heterodimers which multimerize to form a microtubule filament. There are 5 β Tubulin isoforms (β1, β2, β3, β4A and β4B) that are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and ε Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas ε Tubulin localizes to the pericentriolar material. ε Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes.

Tubulin alpha-3C/D chain; Alpha-tubulin 2; Alpha-tubulin 3C/D; Tubulin alpha-2 chain; Detyrosinated tubulin alpha-3C/D chain; TUBA3C; TUBA2; TUBA3D; Tubulin alpha-3E chain; Alpha-tubulin 3E; Detyrosinated tubulin alpha-3E chain; TUBA3E

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of TUBA3C/TUBA3D/TUBA3E polyclonal antibody at 1:500 dilution Lane1:The Brain tissue lysate of Rat (40ug) Lane2:The Brain tissue lysate of Mouse (40ug) Lane3:THP-1 whole cell lysate (40ug) Lane4:K562 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug) Lane6:The Testis tissue lysate of Rat (40ug) Lane7:The Testis tissue lysate of Mouse (40ug)