HSPBAP1 polyclonal antibody

Product Specifications

Background

HSPBAP1 (HSPB (heat shock 27 kDa) associated protein 1), also known as PASS1 (protein associated with small stress protein 1), is a 488 amino acid cytoplasmic protein that contains one JMJC (Jumonji C) domain and shares 80% identity with its rat homolog, PASS1. Widely expressed with highest expression in ovary, thymus and pancreas, HSPBAP1 is thought to play a role in mediating cellular stress responses within the cell. Due to the presence of a JMJC domain, HSPBAP1 may be involved in chromatin remodeling events. Defects or translocations in the gene encoding HSPBAP1 are associated with renal cell carcinoma 1 (RCC1), suggesting a possible role for HSPBAP1 in carcinogenesis. Three isoforms of HSPBAP1 exist due to alternative splicing events.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

HSPB1-associated protein 1; 27 kDa heat shock protein-associated protein 1; Protein associated with small stress protein 1; HSPBAP1; PASS1

Swiss Prot

Q96EW2

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 330-380 of Human HSPBAP1.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HSPBAP1 polyclonal antibody detects endogenous levels of HSPBAP1 protein.

Applications Notes

Western blot (WB) analysis of HSPBAP1 polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug) Lane6:MCF-7 whole cell lysate (40ug) Lane7:A549 whole cell lysate (40ug)