SLC9A8 polyclonal antibody

Product Specifications

Background

Na+/H+ exchangers 1-8, also designated Na+/H+ antiporters or NHE-1-8, are integral membrane proteins expressed in most mammalian tissues where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, NHE isoforms 2-8 have distinct tissue and cell type dependent expression and inhibitory characteristics. NHE-8 is a 575 amino acid protein that localizes apically in intestinal epithelial cells. Expression of NHE-8 is higher in young mammals than adults. NHE-8 gene and protein expression are highly regulated during ontogeny; this protein may play an important role in intestinal Na+ absorption during early mammalian life.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Sodium/hydrogen exchanger 8; Na (+) /H (+) exchanger 8; NHE-8; Solute carrier family 9 member 8; SLC9A8; KIAA0939; NHE8

Swiss Prot

Q9Y2E8

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 450-500 of Human SLC9A8.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 65 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SLC9A8 polyclonal antibody detects endogenous levels of SLC9A8 protein.

Applications Notes

Western blot (WB) analysis of SLC9A8 polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug)