NEK5 polyclonal antibody
Product Specifications
Background
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. Nek5 (NimA-related protein kinase 5) is a 708 amino acid protein that is related to NIMA, a protein that was originally discovered in Aspergillus nidulans and is necessary for entry into mitosis. One of several members of the Ser/Thr protein kinase super family, Nek5 contains one protein kinase domain through which it catalyzes the ATP-dependent phosphorylation of target proteins. Like NIMA, Nek5 may be involved in mitotic regulation and cell cycle control.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Serine/threonine-protein kinase Nek5; Never in mitosis A-related kinase 5; NimA-related protein kinase 5; NEK5
Swiss Prot
Q6P3R8
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to amino acids 150-200 of Human NEK5.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 81 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NEK5 polyclonal antibody detects endogenous levels of NEK5 protein.
Applications Notes
Western blot (WB) analysis of NEK5 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:C0S-7 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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