G3BP2 polyclonal antibody

Product Specifications

Background

Ras GTPase-activating protein-binding protein may be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ras GTPase-activating protein-binding protein 2; G3BP-2; GAP SH3 domain-binding protein 2; G3BP2

Swiss Prot

Q9UN86

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 430-480 of Human G3BP2.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 60, 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

G3BP2 polyclonal antibody detects endogenous levels of G3BP2 protein.

Applications Notes

Western blot (WB) analysis of G3BP2 polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug)