RCC2 polyclonal antibody

Product Specifications

Background

RCC2/TD-60 is a member of the RCC1 (regulator of chromosome condensation 1) family of guanine nucleotide exchange factors. RCC2/TD-60 is associated with the chromosome passenger complex (CPC), which also consists of aurora B kinase, borealin, INCENP (inner centromere protein) and survivin. The CPC acts at various stages of mitosis, interacts with microtubules and is required for proper chromosome segregation and cytokinesis. Regulation of aurora B kinase is key in the regulation of the CPC. In late mitosis, RCC2/TD-60 is required for spindle assembly and recruitment of survivin and aurora B. RCC2/TD-60 is also required for aurora B activation in vitro and localization of the CPC to centromeres.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Protein RCC2; RCC1-like protein TD-60; Telophase disk protein of 60 kDa; RCC2; KIAA1470; TD60

Swiss Prot

Q9P258

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 470-520 of Human RCC2.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RCC2 polyclonal antibody detects endogenous levels of RCC2 protein.

Applications Notes

Western blot (WB) analysis of RCC2 polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug)