DMGDH polyclonal antibody

Product Specifications

Background

DMGDH (dimethylglycine dehydrogenase), also known as ME2GLYDH or DMGDHD, is an 866 amino acid mitochondrial protein that plays a role in choline catabolism by catalyzing the demethylation of dimethylglycine to form sarcosine. Existing as a monomer that belongs to the gcvT family, DMGDH utilizes flavin adenine dinucleotide (FAD) and folate as cofactors. DMGDH is encoded by a gene that maps to human chromosome 5q14.1, defects of which are the cause of DMGDH deficiency (DMGDHD) . Patients with DMGDHD experience muscle fatigue, have a fish-like odor and excrete an elevated level of N, N-dimethylglycine (DMG) in urine.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; DMGDH

Swiss Prot

Q9UI17

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 810-860 of Human DMGDH.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 96 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

DMGDH polyclonal antibody detects endogenous levels of DMGDH protein.

Applications Notes

Western blot (WB) analysis of DMGDH polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug)