WEE2 polyclonal antibody

Product Specifications

Background

Wee1-like protein kinase 2, Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Wee1-like protein kinase 2; Wee1-like protein kinase 1B; Wee1B kinase; WEE2; WEE1B

Swiss Prot

P0C1S8

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding Human WEE2.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 62 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

WEE2 polyclonal antibody detects endogenous levels of WEE2 protein.

Applications Notes

Western blot (WB) analysis of WEE2 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:MEF whole cell lysate (40ug) Lane4:The Testis tissue lysate of Rat (40ug)