MRPL50 polyclonal antibody

Product Specifications

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

39S ribosomal protein L50, mitochondrial; L50mt; MRP-L50; Mitochondrial large ribosomal subunit protein mL50; MRPL50

Swiss Prot

Q8N5N7

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding Human MRPL50.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 18 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MRPL50 polyclonal antibody detects endogenous levels of MRPL50 protein.

Applications Notes

Western blot (WB) analysis of MRPL50 polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug)