MRPL15 polyclonal antibody
Product Specifications
Background
Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-L15 (mitochondrial 39S ribosomal protein L15), also known as L15mt, is a 296 amino acid mitochondrial ribosomal protein belonging to the ribosomal protein L15P family. Localized to mitochondria, MRP-L15 is present in the 39S subunit of the mitoribosomes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
39S ribosomal protein L15, mitochondrial; L15mt; MRP-L15
Swiss Prot
Q9P015
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to amino acids 191-235 of Human MRPL15.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 36 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MRPL15 polyclonal antibody detects endogenous levels of MRPL15 protein.
Applications Notes
Western blot (WB) analysis of MRPL15 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:786-O whole cell lysate (40ug) Lane4:The heart tissue lysate of Mouse (40ug) Lane5:The heart tissue lysate of Rat (40ug)
Host or Source
Rabbit
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