ARL2BP polyclonal antibody

Product Specifications

Background

ADP-ribosylation factors (ARFs) are important in eukaryotic vesicular trafficking pathways and they play an essential role in the activation of phospholipase D (PC-PLD) . ARL2 (ADP-ribosylation factor-like protein 2) functions as a component of a secretory pathway that is involved in the calcium-dependent release of acetylcholine. Additionally, ARL2 plays a role in the folding of tubule proteins, thereby playing an important role in microtubule dynamics and cell cycle progression. BART1 (Binder of ARL2 protein 1), also known as ARL2BP (ADP-ribosylation factor-like protein 2-binding protein), is a 163 amino acid protein that interacts with GTP-bound ARL2 complexes and therefore may play a role in modulating ARL2 activity. Though predominantly cytosolic, BART1 can enter the mitochondria and bind the adenine nucleotide transporter when bound to ARL2. There are two isoforms of BART1 that are produced as a result of alternative splicing events.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ADP-ribosylation factor-like protein 2-binding protein; ARF-like 2-binding protein; ARL2-binding protein; Binder of ARF2 protein 1

Swiss Prot

Q9Y2Y0

Reactivity

Human, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 104-148 of Human ARL2BP.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 100% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 18 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ARL2BP polyclonal antibody detects endogenous levels of ARL2BP protein.

Applications Notes

Western blot (WB) analysis of ARL2BP polyclonal antibody at 1:500 dilution Lane1:Jurkat whole cell lysate (20ug) Lane2:HEK293T whole cell lysate (20ug) Lane3:PC12 whole cell lysate (40ug)