PIKFYVE polyclonal antibody
Product Specifications
Background
The PI (3,5) P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns (3,5) P2) . Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
1-phosphatidylinositol 3-phosphate 5-kinase ; Phosphatidylinositol 3-phosphate 5-kinase; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III; PIPkin-III; Type III PIP kinase
Swiss Prot
Q9Y2I7
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 72-116 of Human PIKFYVE
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Modification
Unmodification
Molecular Weight
~ 260 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PIKFYVE polyclonal antibody detects endogenous levels of PIKFYVE protein.
Applications Notes
Western blot (WB) analysis of PIKFYVE polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:786-O whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:The Testis tissue lysate of Rat (40ug) Lane6:The Testis tissue lysate of Mouse (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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