PIKFYVE polyclonal antibody

Product Specifications

Background

The PI (3,5) P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns (3,5) P2) . Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

1-phosphatidylinositol 3-phosphate 5-kinase ; Phosphatidylinositol 3-phosphate 5-kinase; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III; PIPkin-III; Type III PIP kinase

Swiss Prot

Q9Y2I7

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 72-116 of Human PIKFYVE

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

Modification

Unmodification

Molecular Weight

~ 260 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PIKFYVE polyclonal antibody detects endogenous levels of PIKFYVE protein.

Applications Notes

Western blot (WB) analysis of PIKFYVE polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:786-O whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:The Testis tissue lysate of Rat (40ug) Lane6:The Testis tissue lysate of Mouse (40ug)