APOOL polyclonal antibody

Product Specifications

Background

Apolipoproteins are a family of fatty-acid binding proteins that transport fat through the bloodstream in the form of lipoproteins. ApoO functions to promote the transport of cholesterol from macrophage cells and may be involved in regulatory mechanisms that protect lipid accumulation within the heart. ApoO is present in high density lipoproteins (HDLs) and low density lipoproteins (LDLs), and is secreted by an MTP (microsomal triglyceride transfer protein) -dependent mechanism. ApoOL (apolipoprotein O-like), also known as FAM121A, is a 268 amino acid secreted protein belonging to the apolipoprotein O family and may be involved in cholesterol transport. ApoOL is encoded by a gene located on human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

MICOS complex subunit MIC27; Apolipoprotein O-like; Protein FAM121A

Swiss Prot

Q6UXV4

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 224-268 of Human APOOL

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

Modification

Unmodification

Molecular Weight

~ 34 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

APOOL polyclonal antibody detects endogenous levels of APOOL protein.

Applications Notes

Western blot (WB) analysis of APOOL polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:The Kidney lysate of Rat (40ug) Lane5:The Kidney lysate of Mouse (40ug)