CIDEB polyclonal antibody

The DNA fragmentation factor (DFF) is involved in the caspase-3 apoptotic pathway. DFF is composed of two subunits, a 45 kDa subunit called DFF-45 (also designated ICAD, for inhibitor of CAD), and CPAN (caspase-activated nuclease), also designated CAD (caspase-activated deoxyribonuclease) . CPAN is a DNase that is responsible for DNA degradation during apoptosis. CPAN is inhibited by DFF-45. Caspase-3 acts to dissociate CPAN from DFF-45, allowing CPAN to enter the nucleus and degrade DNA. CIDE-A and CIDE-B have been identified as proteins that share homology with the N-terminal region of DFF-45. Like CPAN, CIDE-A and CIDE-B promote cell death and DNA fragmentation and are inhibited by DFF-45.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

Western blot (WB) analysis of CIDEB polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:The Spleen lysate of Mouse (40ug) Lane4:The Spleen lysate of Rat (40ug)