MRPL22 polyclonal antibody

Product Specifications

Background

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-L22 (mitochondrial ribosomal protein L22), also known as L22mt, RPML25, HSPC158 or MRP-L25, is a 206 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 39S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Belonging to the ribosomal protein L22P family, MRP-L22 is encoded by a gene located on human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

39S ribosomal protein L22, mitochondrial; L22mt; MRP-L22; 39S ribosomal protein L25, mitochondrial; L25mt; MRP-L25

Swiss Prot

Q9NWU5

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 94-138 of Human MRPL22.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 23 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MRPL22 polyclonal antibody detects endogenous levels of MRPL22 protein.

Applications Notes

Western blot (WB) analysis of MRPL22 polyclonal antibody at 1:500 dilution Lane1:THP-1 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)