VGLL2 polyclonal antibody
Product Specifications
Background
Vgl-2 (Transcription cofactor vestigial-like protein 2), also known as VITO-1, is a 317 amino acid protein that contains a domain through which it interacts with TEF-1, a protein that plays a role in controlling the expression of numerous genes. Specific to skeletal muscle, Vgl-2 is expressed highly in adult fast muscle and is expressed at lower levels in adult slow muscle and fetal skeletal muscle. During muscle differentiation, Vgl-2 mRNA levels increase and Vgl-2 translocates from the cytoplasm to the nucleus.Overexpression of Vgl-2 in MYOD-transfected 10T1/2 mouse embryonic fibroblasts increases expression of myosin heavy chain (MHC), which is a marker of terminal muscle differentiation. This evidence suggests that Vgl-2 is essential for muscle gene expression. There are two isoforms of Vgl-2 that are produced as a result of alternative splicing events.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Transcription cofactor vestigial-like protein 2; Vgl-2; Protein VITO1; VGLL2; VITO1
Swiss Prot
Q8N8G2
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 34-79 of Human VGLL2.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 33 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
VGLL2 polyclonal antibody detects endogenous levels of VGLL2 protein.
Applications Notes
Western blot (WB) analysis of VGLL2 polyclonal antibody at 1:500 dilution Lane1:The Heart tissue lysate of Rat (40ug) Lane2:The Heart tissue lysate of Mouse (40ug) Lane3:Hela whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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