ZKSCAN5 polyclonal antibody

Product Specifications

Background

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. A member of the Krüppel C2H2-type zinc-finger protein family, ZKSCAN5, also known as ZFP95, is a 839 amino acid protein containing thirteen C2H2-type zinc fingers, one KRAB A domain and one SCAN box domain. ZKSCAN5 is located in the nucleus and is highly expressed in testis with lower levels of expression in liver, kidney, brain, striated muscle, lung and heart. Many KRAB A box-containing proteins have been shown to function as transcription repressors, suggesting a possible function of ZKSCAN5. As a result of alternative splicing, three transcripts of ZKSCAN5 exist, however one of those transcripts is specific to testis. The mouse homolog of ZKSCAN5 is differentially expressed in spermatogenesis.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ZKSCAN5; Zfp-95; Zinc finger protein with KRAB and SCAN domains 5; Zinc finger protein 95 homolog; KIAA1015; ZFP95

Swiss Prot

Q9Y2L8

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 284-329 of Human ZKSCAN5.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 96 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ZKSCAN5 polyclonal antibody detects endogenous levels of ZKSCAN5 protein.

Applications Notes

Western blot (WB) analysis of ZKSCAN5 polyclonal antibody at 1:500 dilution Lane1:RAW264.7 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug)