TEAD2 polyclonal antibody

Product Specifications

Background

The transcriptional enhancer factor (TEF) /TEAD family includes TEF-1, TEF-3, TEF-4 and TEF-5. These proteins share a highly conserved 68-amino acid TEA/ATTS DNA-binding domain, which binds to SV40 GT-IIC (GGAATG), SphI (AGTATG), SphII (AGCATG), and muscle-specific M-CAT (GGTATG) enhansons. TEFs are differentially expressed in human cultured cell lines and mouse embryonic and extra-embryonic tissues. Specifically, TEF-4 is strongly coexpressed with TEF-1 in mouse mitotic neuroblasts and is also detected in the gut and the nephrogenic region of the kidney. TEF-4 associates with the powerful transcriptional coactivator YAP65 to mediate mitogenic signals. In addition, TEF-4 promotes the activation of the CTP:phosphocholine cytidylyltransferase (CCT) alpha protein, which is the rate-limiting enzyme of phosphatidylcholine biosynthesis, by enhancing the transcriptional activity of Ets-1.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Transcriptional enhancer factor TEF-4; TEA domain family member 2; TEAD-2; TEAD2; TEF4

Swiss Prot

Q15562

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 61-106 of Human TEAD2.

Conjugation

Unconjugated

Related Pathways

Hippo Signaling

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 49 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TEAD2 polyclonal antibody detects endogenous levels of TEAD2 protein.

Applications Notes

Western blot (WB) analysis of TEAD2 polyclonal antibody at 1:500 dilution Lane1:HEH293T whole cell lysate (40ug) Lane2:RAW264.7 whole cell lysat (40ug) Lane3:H9C2 whole cell lysate (40ug)