CD180 polyclonal antibody

Product Specifications

Background

RP105 is a mouse B-cell surface molecule that transmits a growth-promoting signal and is implicated in the life/death decision of B cells. RP105 has tandem repeats of a leucine-rich motif in its extracellular domain that are presumed to be involved in protein-protein interactions. The amino acid sequence of human RP105 is highly homologous to that of mouse RP105; human RP105 shares 74% identity with the mouse protein, as well as the leucine-rich motif. Surface expression of RP105 is enhanced in the presence of MD1, although this expression is restricted to CD19-positive B cells. RP105 demonstrates predominant expression on mature B cells in mantle zones; very little expression is observed in germinal centers.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CD180 antigen; Lymphocyte antigen 64; Radioprotective 105 kDa protein; CD_antigen: CD180; LY64, RP105

Swiss Prot

Q99467

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to residues in Human CD180 .

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 74 kD

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD180 polyclonal antibody detects endogenous levels of CD180 protein.

Applications Notes

Western blot (WB) analysis of CD180 polyclonal antibody at 1:1000 dilution Lane1:HEK293T whole cell lysate (57ug) Lane2:The spleen tissue lysate of Mouse (36ug) Lane3:RAW264.7 whole cell lysate (51ug)