RHD polyclonal antibody

The Rhesus (Rh) blood group system represents one of the most complex and important systems in humans (1) . Two highly homologous genes RHD and RHCE (collectively referred to as RH30 or RHCED) encode the antigens of the Rh blood group system (1–4) . These tightly linked genes map to human chromosomal position 1p34.1-1p36 (2,3) . The RHD gene, which is commonly deleted from a large segment of the population, encodes the most potent blood group immunogen, the D antigen (2,5) . Rh incompatibility between maternal and fetal blood types results in hemolytic disease of the newborn (HDN), which often results in fetal death (1,2,6) . The RHCE gene exists in four allelic forms, and each allele determines the expression of two antigens in Ce, ce, cE, or CE combinations (2,4,5) . The RHCED antigens exist as integral membrane proteins with contain 12-transmembrane helices, and maintain erythrocyte membrane integrity (2) . The presentation of the Rh antigenic activity requires the formation of a complex between the RHCED antigens and RHAG (RH50) (2) .

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of RHD polyclonal antibody at 1:500 dilution Lane1:The Brain tissue lysate of Rat (30ug) Lane2:The Brain tissue lysate of Mouse (30ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)