SRD5A1 polyclonal antibody

Steroid 5α-Reductase is an important enzyme in androgen physiology because it catalyzes the conversion of testosterone into the more potent 5α-dihydro-testosterone, which mediates androgen effects on target tissues. The enzyme exists as two isoforms: type 1, which is expressed mainly in the skin; and type 2, which is expressed mainly in the prostate. In cultured human skin cells, 5α-Reductase 1 shows heterogeneity of protein, and has different levels of transcriptional and translational expression. 5α-Reductase 1 is expressed in all portions of the hair follicle, whereas 5α-Reductase 2 is expressed only in mesenchymal portions. In addition, 5α-Reductase 1 is mainly expressed in human breast carcinoma and may play a role in the in situ production and actions of the potent androgen 5α-dihydrotestosterone, including inhibition of cancer cell proliferation in hormone-dependent human breast carcinoma. The 5α-Reductase-3α-hydroxysteroid dehydrogenase complex is present in the human brain, suggesting that the complex may be involved in the synthesis of neuroactive steroids or the catabolism of neurotoxic steroids.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of SRD5A1 polyclonal antibody at 1:500 dilution Line1:HEK293T whole cell lysate Line2:H9C2 whole cell lysate Line3:Raw264.7 whole cell lysate