Claudin-9 polyclonal antibody

The claudin superfamily consists of many structurally related proteins in humans (1) . These proteins are important structural and functional components of tight junctions in paracellular transport (1,2) . Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues (1) . Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and junction adhesion molecule (3) . Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands (4) . Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions (5) . Claudin-9 is highly similar to claudin-3 (also designated clostridium perfringens enterotoxin receptor) (6) . Claudin-9 is expressed in simian virus (SV) 40-immortalized human corneal epithelial (THCE) cells (7) . The human claudin-9 gene maps to chromosome 16p13.3 (6) .

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of Claudin-9 polyclonal antibody at 1:500 dilution Line1:Hela whole cell lysate Line2:H9C2 whole cell lysate Line3:Raw264.7 whole cell lysate