URB1 polyclonal antibody
Product Specifications
Background
Real-time GlaI-PCR assay is developed to determine DNA methylation status of the regulation regions of HDAC4, URB1 and RARB genes in DNA preparations from human leukocytes. Real-time GlaI-PCR assay is DNA hydrolysis with methyl-directed site-specific DNA endonuclese GlaI followed by real-time PCR from the primers located upstream and downstream of the studied DNA region.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Nucleolar pre-ribosomal-associated protein 1; Nucleolar protein 254 kDa; URB1 ribosome biogenesis 1 homolog; URB1; C21orf108; KIAA0539; NOP254; NPA1
Swiss Prot
O60287
Reactivity
Human
Immunogen
Synthetic peptide, corresponding Human URB1.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 254 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
URB1 pAb detects endogenous levels of URB1 protein.
Applications Notes
Western blot (WB) analysis of URB1 pAb at 1:500 dilution Lane1:Panc1 whole cell lysate (20ug) Lane2:SGC7901 whole cell lysate (20ug) Lane3:MCF-7 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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