DDX24 polyclonal antibody

Product Specifications

Background

DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis and cellular growth and division. DDX24 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 24) is an 859 amino acid protein that contains one helicase C-terminal domain and one helicase ATP-binding domain. Expressed ubiquitously with highest levels in brain and heart, DDX24 functions as an ATP-dependent RNA helicase that is subject to DNA damage-dependent phosphorylation. Human DDX24 shares 79% sequence similarity with its mouse counterpart, suggesting a conserved role between species.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ATP-dependent RNA helicase DDX24 (EC:3.6.4.13) ; DEAD box protein 24; DDX24

Swiss Prot

Q9GZR7

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human DDX24.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 90 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

DDX24 polyclonal antibody detects endogenous levels of DDX24 protein.

Applications Notes

Western blot (WB) analysis of DDX24 polyclonal antibody at 1:500 dilution Line1:A549 whole cell lysate Line2:PC12 whole cell lysate Line3:sp20 whole cell lysate