ATRX polyclonal antibody

Product Specifications

Background

ATRX is a member of the Snf2 family of helicase/ATPases, which contribute to the remodeling of the nucelosome structure in an ATP-dependent manner, and facilitate the initiation of transcription and replication. Structurally, ATRX contains a PHD zinc finger motif. ATRX is regulated throughout the cell cycle where it is differentially distributed within the nucleus. During interphase, ATRX predominately associates with the nuclear matrix, while during mitosis, ATRX localizes with condensed chromatin. At the onset of M phase, phosphorylation rapidly induces this redistribution of ATRX to the short arms of human acrocentric chromosomes, where it then specifically complexes with heterochromatin protein 1 α to mediate chromosomal segregation. Mutations in the ATRX gene correlate with a high incidence of severe X-linked form of syndromal mental retardation associated with α thalassaemia or ATRX syndrome

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Transcriptional regulator ATRX; ATP-dependent helicase ATRX; X-linked helicase II; X-linked nuclear protein; XNP; Znf-HX; ATRX; RAD54L; XH2

Swiss Prot

P46100

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding Human ATRX.

Conjugation

Unconjugated

Related Pathways

ATP-Dependent Chromatin Remodeling Complexes

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 280 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ATRX pAb detects endogenous levels of ATRX protein.

Applications Notes

Western blot (WB) analysis of ATRX pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug) Lane5:EC9706 whole cell lysate (40ug)