MYH6/7 polyclonal antibody

Myosin is a highly conserved, ubiquitously expressed protein that interacts with actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of nonphosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain (MLC) by myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.

Myosin-6; Myosin heavy chain 6; Myosin heavy chain, cardiac muscle alpha isoform; MyHC-alpha; MYH6; MYHCA; Myosin-7; Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform; MyHC-beta; MYH7; MYHCB

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of MYH6/7 polyclonal antibody at 1:500 dilution Lane1:The Heart tissue lysate of Rat (40ug) Lane2:The Heart tissue lysate of Mouse (5ug)