PPP1R13B polyclonal antibody

Product Specifications

Background

ASPP proteins interact with p53 and are responsible for enhancing p53-induced apoptosis but not cell cycle arrest. Inhibition of endogenous ASPP1 (PPP1R13B) function inhibits the apoptotic function of endogenous p53 in response to apoptotic stimuli. ASPP1 amplifies DNA binding and transactivation function of p53 on the promoters of proapoptotic genes in vivo. Expres-sion of ASPP1 is often downregulated in human breast carcinomas expressing wildtype p53, but not in those expressing mutant p53. This research indicates that ASPP1 regulates the tumor suppression function of p53 in vivo. ASPP1 is predominantly a cytoplasmic protein, although some fraction of the polypeptide is nuclear. Defects in PPP1R13B, the gene which encodes ASPP1, may be a cause of breast cancers. The deduced ASPP1 protein contains 1,090 amino acid residues.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Apoptosis-stimulating of p53 protein 1; Protein phosphatase 1 regulatory subunit 13B; PPP1R13B; ASPP1; KIAA0771

Swiss Prot

Q96KQ4

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human PPP1R13B.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 119 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -21°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PPP1R13B polyclonal antibody detects endogenous levels of PPP1R13B protein.

Applications Notes

Western blot (WB) analysis of PPP1R13B polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate