RASSF3 polyclonal antibody

Product Specifications

Background

Ras is a small GTP-binding protein involved in many cellular processes, including proliferation, differentiation and apoptosis. Ras transmits signals of cell surface receptors by binding to a variety of effector molecules. In addition to the well characterized effectors Raf and PI 3-kinase, Ras also interacts with members of the RASSF family, including RASSF1, RASSF2, RASSF3, RASSF4 and Nore1. Members of the RASSF family contain a highly conserved Ras association domain (Ral GDS/AF-6 or RA) and function as Ras effectors/tumor suppressors. RASSF3 (Ras association domain family 3) is a ubiquitously expressed protein found in normal and cancerous tissues. RASSF3 contains an N-terminal RA domain and a coiled-coil SARAH domain. Three isoforms, namely RASSF3A, RASSF3B and RASSF3C, may exist for RASSF3 due to alternative splicing. RASSF3B and RASSF3C are shorter than RASSF3A and do not contain the RA and SARAH domain.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ras association domain-containing protein 3; RASSF3

Swiss Prot

Q86WH2

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human RASSF3.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 28 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RASSF3 polyclonal antibody detects endogenous levels of RASSF3 protein.

Applications Notes

Western blot (WB) analysis of RASSF3 polyclonal antibody at 1:500 dilution Lane1:Mcf-7 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate