EDEM1 polyclonal antibody

Product Specifications

Background

Proteins expressed in the endoplasmic reticulum (ER) are subjected to a tight quality control. Terminally misfolded proteins in the endoplasmic reticulum (ER) are retrotranslocated to the cytoplasm and degraded by proteasomes through a mechanism known as ER-associated degradation (ERAD) . EDEM (ER degradation-enhancing alpha-mannosidase-like) protein is a type II membrane protein that localizes to the ER and is directly involved in ERAD. EDEM targets misfolded glycoproteins for degradation in an N-glycan-dependent manner and extracts misfolded glycoproteins from the calnexin cycle. The human EDEM gene maps to chromosome 3p.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ER degradation-enhancing alpha-mannosidase-like protein 1; EDEM1; EDEM; KIAA0212

Swiss Prot

Q92611

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human EDEM1

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 74 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

EDEM1 polyclonal antibody detects endogenous levels of EDEM1 protein.

Applications Notes

Western blot (WB) analysis of EDEM1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate