PODXL2 polyclonal antibody

Product Specifications

Background

Endoglycan, also known as podocalyxin-like 2, exists as a disulfide-dependent homodimer and belongs to the CD34 family of proteins. It is a proteoglycan modified with chondroitin sulfate chains and (with appropriate post-translational modifications) functions as an L-selectin ligand, interacting via sulfation on two tyrosine residues. Endoglycan contains a membrane proximal globular domain, a single pass transmembrane domain, a mucin-like domain and a highly acidic N-terminal with O-linked sLex structures. It is widely expressed with distribution including endothelial cells, hematopoietic precursors and leukocyte subpopulations. Endoglycan is similar to PSGL-1 in that it also exhibits catch-slip transitional bonds. These bonds help to mediate the rolling and tethering of circulating leukocytes on vascular surfaces, typically during immune surveillance and inflammation. The ligand activity of Endoglycan is dependent on sialylation and fucosylation and may mediate adhesion events.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Podocalyxin-like protein 2; Endoglycan; PODXL2; UNQ1861; PRO3742

Swiss Prot

Q9NZ53

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human PODXL2

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 65 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PODXL2 polyclonal antibody detects endogenous levels of PODXL2 protein.

Applications Notes

Western blot (WB) analysis of PODXL2 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate