INDO polyclonal antibody

Product Specifications

Background

Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible enzyme that catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. The gene encoding human IDO maps to chromosome 8p12-p11. IDO, also known as INDO, is an important modulator of immunological responses and protects allogeneic concepti from alloreactive maternal lymphocytes. IDO mediates an interesting inhibitory effect of HeLa cells co-cultured with human PBLs. The ILN-2-induced proliferation response of PBLs is diminished in the presence of HeLa cells while an IDO inhbitor negates this effect. Flow cytometric analysis indicates both mature and immature CD123-positive dentritic cells suppress T cell activity using IDO. IDO-transfected cells co-cultured with T cells reduces T cell proliferation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Indoleamine 2,3-dioxygenase 1; IDO-1; Indoleamine-pyrrole 2,3-dioxygenase; IDO1; IDO; INDO

Swiss Prot

P14902

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human INDO.

Conjugation

Unconjugated

Related Pathways

Immune Cell Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 45 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

INDO polyclonal antibody detects endogenous levels of INDO protein.

Applications Notes

Western blot (WB) analysis of INDO polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with IFNγ (100ug/ml,15mins) Lane2:A549 cell lysate treated with IFNγ (100ug/ml,15mins) Lane3:H9C2 cell lysate treated with IFNγ (100ug/ml,15mins)