RAB14 polyclonal antibody

Product Specifications

Background

The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies all of which are thought to play an important role in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum (ER) to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab proteins are also an integral part of endocytic pathways. Rab 14, also known as FBP, is a 215 amino acid protein that is lipid-anchored to the cytoplasmic side of the cell membrane. One of several members of the Rab subfamily of small GTPases, Rab 14 is thought to be involved in vesicular trafficking and neurotransmitter release throughout the body and is expressed at high levels in brain, lung, kidney, spleen and thymus.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ras-related protein Rab-14; RAB14

Swiss Prot

P61106

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human RAB14.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 22 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RAB14 polyclonal antibody detects endogenous levels of RAB14 protein.

Applications Notes

Western blot (WB) analysis of RAB14 polyclonal antibody at 1:500 dilution Lane1:Jurkat whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate