Profilin-2 polyclonal antibody

Product Specifications

Background

Profilins (Profilin-1, Profilin-2 and Profilin-3) act as nucleotide exchange factors that use ATP to charge Actin, a process that regulates Actin polymerization by subsequently sequestering the Actin monomer. Profilin-2, also known as PFN2 or PFL, is a 140 amino acid protein that is ubiquitously expressed with highest expression in kidney, brain and skeletal muscle. Like other members of the Profilin family, Profilin-2 functions as an Actin monomer-binding protein that influences the structure of the cytoskeleton by regulating Actin polymerization in response to extracellular signals. High levels of Profilin-2 inhibit Actin polymerization, while lower levels enhance Actin polymerization, suggesting that the expression of Profilin-2 is regulated by the need for polymerization within the cell. Two isoforms of Profilin-2 exist due to alternative splicing events.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Profilin2; Profilin 2; Profilin II; PFN2

Swiss Prot

P35080

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human Profilin-2.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 15 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Profilin-2 polyclonal antibody detects endogenous levels of Profilin-2 protein.

Applications Notes

Western blot (WB) analysis of Profilin-2 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Mouse brain tissue lysate Lane3:Rat skeletal muscle tissue lysate