HES-2 polyclonal antibody

Product Specifications

Background

The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG) . HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues. HES3 is expressed exclusively in cerebellar Purkinje cells, and HES5 is found solely in the nervous system. HES6 is produced in brain as well as in the limb buds of developing embryos.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Transcription factor HES-2; Class B basic helix-loop-helix protein 40; bHLHb40; Hairy and enhancer of split 2; HES2; BHLHB40

Swiss Prot

Q9Y543

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human HES-2.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 18 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HES-2 polyclonal antibody detects endogenous levels of HES-2 protein.

Applications Notes

Western blot (WB) analysis of HES-2 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Mouse brain tissue lysate Lane3:Rat brain tissue lysate