MAL polyclonal antibody
Product Specifications
Background
The MAL-SRF interaction displays the predicted properties of a Rho-regulated SRF cofactor. MAL is predominantly cytoplasmic in serum-starved cells, but accumulates in the nucleus following serum stimulation. Activation of the Rho-actin signaling pathway is necessary and sufficient to promote MAL nuclear accumulation. MAL N-terminal sequences, including two RPEL motifs, are required for the response to signaling, while other regions mediate its nuclear export (or cytoplasmic retention) and nuclear import. MAL associates with unpolymerized actin through its RPEL motifs. Constitutively cytoplasmic MAL derivatives interfere with MAL redistribution and Rho-actin signaling to SRF.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Myelin and lymphocyte protein; T-lymphocyte maturation-associated protein; MAL
Swiss Prot
P21145
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding Human MAL.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 16 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MAL pAb detects endogenous levels of MAL protein.
Applications Notes
Western blot (WB) analysis of MAL pAb at 1:500 dilution Lane1:Myla2059 whole cell lysate (40ug) Lane2:HuT78 whole cell lysate (40ug) Lane3:The Spleen tissue lysate of Rat (40ug) Lane4:The Spleen tissue lysate of Mouse (40ug) Lane5:AML-12 whole cell lysate (40ug) Lane6:CT26 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
