MED29 polyclonal antibody
Product Specifications
Background
In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II) . The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med29 (mediator complex subunit 29), also known as IXL (intersex-like), is a 200 amino acid nuclear protein and component of the mediator complex. Widely expressed in embryo and adult tissue, Med29 is considered a novel amplification target gene in pancreatic cancer and is encoded by a gene that maps to human chromosome 19q13.2. Chromosome 19 consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Mediator of RNA polymerase II transcription subunit 29; Intersex-like protein; Mediator complex subunit 29; MED29; IXL
Swiss Prot
Q9NX70
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human MED29.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 24 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MED29 polyclonal antibody detects endogenous levels of MED29 protein.
Applications Notes
Western blot (WB) analysis of MED29 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Mouse brain tissue lysate Lane3:Rat lung tissue lysate
Host or Source
Rabbit
Available Sizes
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