CABYR polyclonal antibody

Spermatozoa gain fertilization capacitation and hyperactivation after residing in the uterus or oviduct. Calcium, cAMP and protein tyrosine phosphorylation are necessary for the molecular mechanisms that allow for this transformation. CABYR (Calcium-binding tyrosine phosphorylation-regulated protein), also known as Fibrousheathin-2 or Cancer/testis antigen 88, is a 493 amino acid protein that is expressed in sperm flagella and exhibits increased tyrosine phosphorylation during capacitation. There are six named isoforms of CABYR that are produced as a result of alternative slicing events. Specifically, isoform 1 is expressed in the testis, while isoform 3 and isoform 5 are expressed in pancreas, brain and various brain tumors. Isoforms 1, 2 and 6 most likely bind calcium, whereas isoforms 3, 4 and 5 probably do not bind calcium.

Calcium-binding tyrosine phosphorylation-regulated protein; Calcium-binding protein 86; Cancer/testis antigen 88; CT88; Fibrousheathin II; Fibrousheathin-2; FSP-2; Testis-specific calcium-binding protein CBP86; CBP86; FSP2

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of CABYR polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate