AXIN1 polyclonal antibody

Product Specifications

Background

β-catenin is a component of both the cadherin cell adhesion system and the Wnt signaling pathway. Wnt signaling increases the amount of β-catenin by preventing its ubiquitination and degradation, allowing its direct interaction with transcription factors of the lymphoid enhancer factor/T cell factor family, and modulation of gene expression. Axin is involved in the degradation of β-catenin by acting as a scaffold to form a complex between β-catenin, adenomatous polyposis coli (APC) and GSK-3β. APC, which is phosphorylated by GSK-3β, induces degradation of β-catenin, thus inhibiting Wnt signal transduction. Conductin is 45% identical to Axin and appears to play a similar role to Axin in the Wnt signaling pathway.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Axin-1; Axis inhibition protein 1; hAxin; AXIN1; AXIN

Swiss Prot

O15169

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human AXIN1.

Conjugation

Unconjugated

Related Pathways

Wntβ catenin signaling, Contribution of Soluble Factors to EMT

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 110 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AXIN1 polyclonal antibody detects endogenous levels of AXIN1 protein.

Applications Notes

Western blot (WB) analysis of AXIN1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate