RECQL4 polyclonal antibody

Product Specifications

Background

In humans, the RecQ helicase family includes WRN, BLM, RecQL1, RecQL4 and RecQL5 proteins, all of which contain a conserved helicase domain. WRN and BLM have been demonstrated to be the responsible genes in Werner and Bloom syndromes, respectively. RecQL1 and RecQL5 also belong to the human RecQ helicase family, but their correlation with genetic disorders, if any, is unknown. The gene encoding human RecQL4, which maps to chromosome 8q24.3, is believed to be the gene responsible for the development of Rothmund-Thomson syndrome (RTS) . The levels of WRN, BLM, RecQL1, 4 and 5 are differentially up-regulated to guarantee genomic stability in cells that are transformed or actively proliferating. In humans, RecQL1 and RecQL5 map to chromosome 12p12 and 17q25, respectively.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ATP-dependent DNA helicase Q4; DNA helicase, RecQ-like type 4; RecQ4; RTS; RecQ protein-like 4; RECQ4

Swiss Prot

O94761

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human RECQL4.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 133 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RECQL4 polyclonal antibody detects endogenous levels of RECQL4 protein.

Applications Notes

Western blot (WB) analysis of RECQL4 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:Rat brain tissue lysate