ATE1 polyclonal antibody
Product Specifications
Background
Arginyl-tRNA-protein transferase (ATE1), also designated arginyltransferase 1, belongs to the R-transferase family of proteins. In order for a protein to be degraded via the ubiquitin pathway, arginylation of the protein is required. ATE1 plays an important role in this process, as it is important for the posttranslational conjugation of arginine to the N-terminal aspartate-, glutamate- and possibly cystine-contaning substrates. ATE1 is a 518 amino acid protein. Alternative splicing results in two distinct isoforms. ATE1, which is found as a monomer, can localize to the cytoplasm and/or the nucleus.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Arginyl-tRNA--protein transferase 1; Arginyltransferase 1; R-transferase 1; Arginine-tRNA--protein transferase 1
Swiss Prot
O95260
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human ATE1.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 59 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ATE1 polyclonal antibody detects endogenous levels of ATE1 protein.
Applications Notes
Western blot (WB) analysis of ATE1 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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