ACER1 polyclonal antibody
Product Specifications
Background
ASAH3 (N-acylsphingosine amidohydrolase (alkaline ceramidase) 3), also designated ACER1, is a 264 amino acid multi-pass membrane protein that localizes to the membrane of the endoplasmic reticulum. Expressed predominately in epidermal tissue, ASAH3 catalyzes the hydrolysis of the sphingolipid ceramide into sphingosine and a free fatty acid, thereby playing an important role in the regulation of bioactive ceramide and sphingosine levels within the cell. ASAH3 functions at an optimal pH of 8.0 and has high specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine, but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Alkaline ceramidase 1; AlkCDase 1; Alkaline CDase 1; EC=3.5.1.23; Acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3; ACER1; ASAH3
Swiss Prot
Q8TDN7
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human ACER1
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ACER1 polyclonal antibody detects endogenous levels of ACER1 protein.
Applications Notes
Western blot (WB) analysis of ACER1 polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
Host or Source
Rabbit
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