CD52 polyclonal antibody
Product Specifications
Background
CD52 is a glycosylphosphatidylinositol (GPI) -linked surface antigen present at high levels on epithelial cells lining the male reproductive tract, thymocytes, lymphocytes, monocytes and macrophages. It is also present at variable levels on lymphoid malignancies. During sperm maturation, spermatozoa passing through the genital tract acquire CD52 that is shed from the epithelial cell lining into seminal plasma. CD52 is detectable on the surface of epididymal sperm and in the ejaculate but not on spermatogenetic cells or testicular spermatozoa. The peptide backbone of CD52, which consists of 12 amino acids, is considered a mere scaffold for posttranslational modifications, such as GPI-anchor and N-glycosylation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
CAMPATH-1 antigen; CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; He5; CD52; CD52; CDW52, HE5
Swiss Prot
P31358
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human CD52.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 15 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CD52 polyclonal antibody detects endogenous levels of CD52 protein.
Applications Notes
Western blot (WB) analysis of CD52 polyclonal antibody at 1:500 dilution Lane1:THP-1 whole cell lysate Lane2:Mouse spleen tissue lysate Lane3:H9C2 whole cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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