RPL30 polyclonal antibody
Product Specifications
Background
Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L30, also known as RPL30, is a 115 amino acid protein that localizes to the cytoplasm and exists as a component of the 60S subunit, possibly playing a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L30 exists as multiple processed pseudogenes that are scattered throughout the genome. The gene encoding Ribosomal Protein L30 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
60S ribosomal protein L30
Swiss Prot
P62888
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to the 70-115 of Human RPL30.
Conjugation
Unconjugated
Applications
WB IHC ICC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 13 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
RPL30 polyclonal antibody detects endogenous levels of 60S ribosomal protein L30 protein.
Applications Notes
Western blot (WB) analysis of RPL30 pAb at 1:500 dilution Lane1:DLD whole cell lysate (20ug) Lane2:H1792 whole cell lysate (20ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Brain tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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