RAB33A polyclonal antibody

Product Specifications

Background

The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies. Increasing data suggests an important role for Rab proteins in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab proteins are also an integral part of endocytic pathways. Rab 33B and Rab 33A share conserved effector domains, but share only 55.3% overall amino acid identity. This suggests that Rab 33B and Rab 33A may interact with similar effector and regulatory proteins.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ras-related protein Rab-33A; Small GTP-binding protein S10; RAB 33A; RABS10

Swiss Prot

Q14088

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 173-218 of Human RAB33A.

Conjugation

Unconjugated

Applications

WB ICC

Dilution

WB: 1:500~1:1000 ICC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 27 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RAB33A polyclonal antibody detects endogenous levels of RAB33A protein.

Applications Notes

Western blot (WB) analysis of RAB33A polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:PC12 cell lysate