PPP2R1B polyclonal antibody

Product Specifications

Background

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2A family comprises subfamily members PP2Aα and PP2Aβ. The PP2A catalytic subunit associates with a variety of regulatory subunits. Regulatory subunits include PP2A-Aα and -Aβ, PP2A-Bα and -Bβ, PP2A-Cα and -Cβ, and PP2A-B56α and -B56β.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta; PP2A-Aβ; PP2AAβ; PP2A Aβ; PP2A-APR65β; PP2A -AR1β

Swiss Prot

P30154

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 556-601 of Human PPP2R1B.

Conjugation

Unconjugated

Applications

WB IHC ICC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 66 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PPP2R1B polyclonal antibody detects endogenous levels of PPP2R1B protein.

Applications Notes

Western blot (WB) analysis of PP2A-Aβ (F590) pAb at 1:1000 dilution Lane1:A2780 whole cell lysate (40ug) Lane2:Beas-2B whole cell lysate (40ug) Lane3:Myla2059 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug)