PITPNB polyclonal antibody

Product Specifications

Background

The lipid binding proteins known as phosphatidylinositol transfer proteins (PITP) facilitate the formation of phosphatidylinositol derived second messenger molecules, which are related to the phospholipase C and phosphoinositide 3-kinase pathways. PITP are ubiquitously expressed proteins that transfer phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes enriched in PI or PC to membranes that are deficient in PI or PC. PITP mobilizes PI from the endoplasmic recticulum and regulates the release of PI from stored vesicles in the Golgi network. In mammalian cells, three smaller forms of soluble PITP are present, designated PITPα, PITPβ and retinal degeneration B (rdgB) beta. PITPβ is a 271 amino acid protein that is widely expressed in various tissues. Though required for Golgi targeting, constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity. There are two isoforms of PITPβ that are produced as a result of alternative splicing events.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Phosphatidylinositol transfer protein beta isoform; PI-TP-beta; PtdIns transfer protein beta; PtdInsTP beta; PITPβ; PI-TPβ; PI-TP-β

Swiss Prot

P48739

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 226-271 of Human PITPNB.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 32 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PITPNB polyclonal antibody detects endogenous levels of PITPNB protein.

Applications Notes

Western blot (WB) analysis of PITPNB polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:Rat heart tissue lysate