ARHGEF5 polyclonal antibody
Product Specifications
Background
Rho GTPases, which play fundamental roles in numerous cellular processes, are initiated by external stimuli that signal though G-protein coupled receptors. ARHGEF5 (Rho guanine nucleotide exchange factor (GEF) 5), also known as P60 or transforming immortalized mammary oncogene (TIM), is a 1597 amino acid cytoplasmic protein. ARHGEF5 is believed to form a complex with G proteins and be involved in the initiation of Rho-dependent signals. It has also been suggested that ARHGEF5 may be involved in the organization of the cytoskeleton. ARHGEF5 contains one PH domain, one DH domain and one SH3 domain. Existing as two isoforms produced by alternative splicing, ARHGEF5 is mainly expressed in pancreas, liver, kidney, placenta and lung.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Rho guanine nucleotide exchange factor 5; Ephexin-3; Ephexin 3; Ephexin3; Guanine nucleotide regulatory protein TIM; Oncogene TIM; Transforming immortalized mammary oncogene; p60 TIM; TIM
Swiss Prot
Q12774
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 1310-1355 of Human ARHGEF5.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 60, 177 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ARHGEF5 polyclonal antibody detects endogenous levels of ARHGEF5 protein.
Applications Notes
Western blot (WB) analysis of ARHGEF5 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate
Host or Source
Rabbit
Available Sizes
Curated Selection
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